인제대 / 박진희, 박세광*
Abstract
Purpose: The establishment of a preclinical model of the abscopal effect on HCC and evaluation on whether the hypofractionated RT multi-tumor Hepa1-6 mouse HCC model could be used to suppress non-radiated tumor mass would be implemented in this study.
Methods and materials: Hepa1-6 mouse liver cancer cell lines were used to form tumors and immunogenicity was analyzed using ELISpot and immune cell labeled antibody. Interferonβ (IFNβ) expression was confirmed through PCR.
Results: After investigation, the intratumoral transcription of type Ⅰ IFN increased by 2-fold. The anti-tumor immune response to Hepa 1-6 cells induced by radiation was increased. Moreover, the influx of activated CD8+ T cell was increased in non-irradiated tumors. The number of dendritic cells and activation status were evaluated by flow cytometry on the second day after irradiation. The flow cytometry presented significantly increased DC population expressing the CD11c molecule in tumor draining lymph nodes. Furthermore, as the irradiation leads to adaptation of immune resistance of tumor cells against RT, we explored to elucidate potent tool to overcome the resistance and confirmed the capability of PD-L1 antibody to survive the late RT resistance.
Conclusions: The immunological mechanism of the abscopal effect was revealed and the application of PD-L1 inhibitor successfully performed as a breakthrough to the late RT resistance in Hepa1-6 tumor model.
방사선치료에 따른 abscopal 효과의 발생과 면역관문 억제 항체 치료의 병용에 따른 치료효과 증가 결과
방사선치료에 따른 abscopal 효과의 발생 기전
Affiliations
Jin Hee Park 1 , Hee Yeon Kim 2 , Anbok Lee 2 , Young Kyeong Seo 3 , Il-Hwan Kim 4 , Eun-Tae Park 5 , Mi Seon Kang 6 , Sung Jae Park 7 , SaeGwang Park 8
1 Department of Microbiology, College of Medicine, Inje University, Busan, Republic of Korea.
2 Departments of Surgery, Busan Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea.
3 Departments of Internal Medicine, Busan Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea.
4 Departments of Internal Medicine, Haeundae Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea.
5 Departments of Radiation Oncology, Busan Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea.
6 Departments of Pathology, Busan Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea.
7 Departments of Internal Medicine, Busan Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea. Electronic address: yaheaven@inje.ac.kr.
8 Department of Microbiology, College of Medicine, Inje University, Busan, Republic of Korea. Electronic address: micpsg@inje.ac.kr.
편집위원
본 연구는 HCC에 대한 abscopal effect의 preclinical model 확립을 통해 abscopal effect의 면역학적 기전을 규명하였으며, PD-L1 inhibitor가 late RT resistance에 매우 효과적임을 증명한 내용이다.
2021-01-26 15:20:01