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Abstract
Since ancient times, various herbs have been used in Asia, including Korea, China, and Japan, for wound healing and antiaging of the skin. In this study, we manufactured and chemically analyzed a novel distillate obtained from a fermented mixture of nine anti-inflammatory herbs (Angelica gigas, Lonicera japonica, Dictamnus dasycarpus Turcz., D. opposita Thunb., Ulmus davidiana var. japonica, Hordeum vulgare var. hexastichon Aschers., Xanthium strumarium L., Cnidium officinale, and Houttuynia cordata Thunb.). The fermentation of natural plants possesses beneficial effects in living systems. These activities are attributed to the chemical conversion of the parent plants to functional constituents which show more potent biological activities. In our current study, the distillate has been manufactured after fermenting the nine oriental medical plants with Lactobacillus fermentum, followed by distilling. We analyzed the chemical ingredients involved in the distillate and evaluated the effects of topical application of the distillate on ultraviolet B (UVB)-induced skin damage in Institute of Cancer Research (ICR) mice. Topical application of the distillate significantly ameliorated the macroscopic and microscopic morphology of the dorsal skin against photodamage induced by UVB radiation. Additionally, our current results showed that topical application of the distillate alleviated collagen disruption and reduced levels of proinflammatory cytokines (tumor necrosis factor alpha and interleukin 1 β expressions) in the dorsal skin against UVB radiation. Taken together, our current findings suggest that the distillate has a potential to be used as a material to develop a photoprotective adjuvant.

Affiliations

Young Her  1 , Tae-Kyeong Lee  2 , Ji Hyeon Ahn  3   4 , Soon Sung Lim  5 , Beom-Goo Kang  6 , Jung-Seok Park  7 , Bora Kim  3 , Hyejin Sim  3 , Jae-Chul Lee  3 , Hyun Sook Kim  8 , Tae Heung Sim  8 , Hyun Sam Lee  8 , Moo-Ho Won  3
1 Department of Dermatology, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Gangwon 24289, Korea.
2 Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Korea.
3 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea.
4 Department of Physical Therapy, College of Health Science, Youngsan University, Yangsan, Gyeongnam 50510, Korea.
5 Department of Food Science and Nutrition, College of Natural Sciences, Hallym University, Chuncheon 24252, Korea.
6 Department of Biochemistry, College of Medicine, Hallym University, Chuncheon 24252, Korea.
7 Department of Physical Education, College of Natural Science, Hallym University, Chuncheon 24252, Korea.
8 Leefarm Co., Ltd., Hongcheon, Gangwon 25117, Korea.