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Abstract

In this study, we developed a pore size/pore area-controlled optical biosensor-based anodic aluminum oxide (AAO) nanostructure. As the pore size of AAO increases, the unit cell of AAO increases, which also increases the non-pore area to which the antibody binds. The increase in the number of antibodies immobilized on the surface of the AAO enables effective detection of trace amounts of antigen, because increased antigen-antibody bonding results in a larger surface refractive index change. High sensitivity was thus achieved through amplification of the interference wave of two vertically-incident reflected waves through the localized surface plasmon resonance phenomenon. The sensitivity of the fabricated sensor was evaluated by measuring the change in wavelength with the change in the refractive index of the device surface, and sensitivity was increased with increasing pore-size and non-pore area. The sensitivity of the fabricated sensor was improved and up to 11.8 ag/mL serum amyloid A1 antigen was detected. In addition, the selectivity of the fabricated sensor was confirmed through a reaction with a heterogeneous substance, C-reactive protein antigen. By using hard anodization during fabrication of the AAO, the fabrication time of the device was reduced and the AAO chip was fabricated quickly and easily. 

Author information

Kim SW1, Lee JS2, Lee SW3, Kang BH4, Kwon JB5, Kim OS6, Kim JS7, Kim ES8, Kwon DH9, Kang SW10.​

1School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. kei95304@gamil.com.2School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. jslee_1245@naver.com.3School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. sw3148@ee.knu.ac.kr.4Division of Advanced Research and Development, SINOKOR, 12 Seongseogongdanbuk-ro 43-gil, Dalseo-gu, Daegu 704-920, Korea. bhkang@ee.knu.ac.kr.5School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. jinbumkwon@naver.com.6School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. oskim@knu.ac.kr.7School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. jskim5772@ee.knu.ac.kr.8Division of Computer and Electronic Engineering, Pusan University of Foreign studies, 65 Namsan-dong, Geumjeong-gu, 608-738 Busan, Korea. eskim@pufs.ac.kr.9Department of Electronics Engineering, Kyungil University, Hayang-up, Gyeongsang buk-do 712-702, Korea. dhkwon@kiu.ac.kr.10School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea. swkang@knu.ac.kr.