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  • 2021년 05월호
    [Stem Cells.] Extracellular vesicles derived from fibroblasts promote wound healing by optimizing fibroblast and endothelial cellular functions

    경북의대 / 오은정, 정호윤*, 안병철*

  • 출처
    Stem Cells.
  • 등재일
    2021 Mar
  • 저널이슈번호
    39(3):266-279. doi: 10.1002/stem.3310. Epub 2020 Dec 31.
  • 내용

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    Abstract
    Extracellular vesicles (EVs) have been exhibited as promising candidates for delivering endogenous therapeutic cargos for regenerative therapies. Fibroblasts could be candidate source cells for EVs, to investigate their therapeutic effects in wound healing. Here we demonstrated the isolation and characterization of fibroblast-derived (L929 cell line) EVs (L929-EVs). Furthermore, L929-EVs treatment showed pro-wound healing effects in vitro by enhancing proliferation, migration, and scarless wound healing related genes in fibroblast cells. L929-EVs treatment also enhanced the migration and tube formation of endothelial cells. The combination of L929-EVs with fibrin glue accelerated wound healing in the mouse skin wound model by enhancing collagen formation, collagen maturation, and blood vessels in the wounded skin. The role of fibroblast-derived EVs in wound healing could be an important phenomenon, and fibroblast-derived EVs could be harnessed for wound healing therapies.

     

     

    Affiliations

    Eun Jung Oh  1 , Prakash Gangadaran  2   3 , Ramya Lakshmi Rajendran  2 , Hyun Mi Kim  1 , Ji Min Oh  2 , Kang Young Choi  1 , Ho Yun Chung  1   3 , Byeong-Cheol Ahn  2   3   4
    1 Department of Plastic and Reconstructive Surgery, CMRI, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Republic of Korea.
    2 Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
    3 BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
    4 Department of Nuclear Medicine, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.

  • 키워드
    extracellular vesicles; mouse model; wound healing; wound repair.
  • 편집위원

    섬유아세포에서 분리된 세포외 소포가 손상된 피부조직의 회복에 도움이 보여준 연구임. 세포외 소포 관련 연구자 및 상처 회복 개선에 관심이 있는 피부과 및 성형외과 관련 임상가 에게 관심을 끌 연구로 생각됨.

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