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  • [Cells .] FITC-Labeled RGD Peptides as Novel Contrast Agents for Functional Fluorescent Angiographic Detection of Retinal and Choroidal Neovascularization
    망막 및 맥락막 혈관신생의 기능적 형광 혈관조영 검출을 위한 새로운 조영제로서의 FITC 표지 RGD 펩티드

    서울대 / 최승우, 홍혜경, 이병철*, 우세준*

  • 출처
    Cells .
  • 등재일
    2023 Jul 21
  • 저널이슈번호
    12(14):1902. doi: 10.3390/cells12141902.
  • 내용

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    Abstract
    The development of choroidal neovascularization (CNV) is a crucial factor in the pathophysiology and prognosis of exudative age-related macular degeneration (AMD). Therefore, the detection of CNV is essential for establishing an appropriate diagnosis and treatment plan. Current ophthalmic imaging techniques, such as fundus fluorescent angiography and optical coherence tomography, have limitations in accurately visualizing CNV lesions and expressing CNV activity, owing to issues such as excessive dye leakage with pooling and the inability to provide functional information. Here, using the arginine-glycine-aspartic acid (RGD) peptide's affinity for integrin αvβ3, which is expressed in the neovascular endothelial cells in ocular tissues, we propose the use of fluorescein isothiocyanate (FITC)-labeled RGD peptide as a novel dye for effective molecular imaging of CNV. FITC-labeled RGD peptides (FITC-RGD2), prepared by bioconjugation of one FITC molecule with two RGD peptides, demonstrated better visualization and precise localization of CNV lesions than conventional fluorescein dyes in laser-induced CNV rodent models, as assessed using various imaging techniques, including a commercially available clinical fundus camera (Optos). These results suggest that FITC-RGD2 can serve as an effective novel dye for the diagnosis of neovascular retinal diseases, including AMD, by enabling early detection and treatment of disease occurrence and recurrence after treatment.

     

     

    Affiliations

    Seung Woo Choi 1, Hye Kyoung Hong 1, Jehwi Jeon 2 3, Ji Young Choi 4 5, Minah Kim 1, Pilhan Kim 2 3, Byung Chul Lee 4, Se Joon Woo 1
    1Department of Ophthalmology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
    2Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
    3KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
    4Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
    5Bio-Max Institute, Seoul National University, Seoul 08826, Republic of Korea.

  • 키워드
    RGD peptides; age-related macular degeneration; choroidal neovascularization; contrast agents; fundus fluorescent angiography.
  • 편집위원

    맥락막 혈관신생(CNV)는 맥락막(choroid)에서 발생하는 병적 혈관으로삼출형 연령관련 황반변성(AMD)의 예후에 중요한 요소로 알려져 있기 때문에, CNV의 검출은 적절한 진단 및 치료 계획 수립에 필수적이다. 따라서 이 논문은 CNV의 효과적인 검출을 위해, 안구 조직의 신생혈관 내피 세포에서 발현되는 인테그린 αvβ3에 대한 arginine-glycine aspartic acid (RGD) 펩타이드의 친화력을 바탕으로 하는 fluorescein isothiocyanate (FITC)가 표지된 2중 RGD 펩타이드(FITC-RGD2) 개발을 보여주며 기존보다 향상된 CNV 병변의 표적 결과를 동물실험을 통해서 제시하고 있다.

    2023-09-06 14:27:02

  • 편집위원2

    맥락막 혈관신생(CNV)의 보다 정확한 진단을 위해 형광물질로 표지한 RGD peptides를 이용하여 기존의 fluorescein을 이용한 안구의 혈관조영술의 단점을 개선하고자 함. 수치적으로 뚜렷한 개선을 보여주지는 않으나, 향후 맥락막 신생혈관의 감지로 적절한 진단과 치료 계획 수립에 이용될 수 있는 새로운 영상 기술의 필요성을 제시함.

    2023-09-06 14:27:34

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