글로벌 연구동향
분자영상 및 방사화학
- 2019년 03월호
[Angew Chem Int Ed Engl.] 살아있는 세포에서 수용체 올리고머화의 직접적인 시각화를 위한 분자형광법 Homo-molecular Fluorescence Complementation for Direct Visualization of Receptor Oligomerization in Living Cells.KAIST / 송대선, 정용원*
- 출처
- Angew Chem Int Ed Engl.
- 등재일
- 2019 Feb 11
- 저널이슈번호
- 58(7):2045-2049. doi: 10.1002/anie.201812780. Epub 2019 Jan 14.
- 내용
Abstract
Cell surface receptor oligomerization is an attractive target process for drug screening. However, simple but reliable (and thus high-throughput) visualization methods for receptor oligomerization are still lacking. Herein, we report on a new single-construct homo-molecular fluorescence complementation (Homo-FC) probe, which shows strong fluorescence signals by oligomerization of fused receptors in living cells with unexpectedly low background signals. Importantly, this high signal-to-noise ratio was not affected by expression level variations of fused receptors. The Homo-FC probe was developed by optimized flopped fusion of split fragments of superfolder green fluorescence protein and subsequent surface charge engineering. Homo-FC reliably visualized the oligomerization of diverse natural receptors such as GPCR, EGFR, and even cytosolic DAI.
Author informationSong D1, Jung Y1.
1
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
- 키워드
- drug discovery; fluorescence complementation; imaging; membrane receptor oligomerization; protein engineering
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