고려대 / 임채홍, 김철용*
Abstract
INTRODUCTION:
Radioprotectors can enhance the efficacy of cancer radiotherapy, but their clinical use remains uncommon. The present study aimed to assess the radioprotective potential of mistletoe extract (commercial name: Abnoba Viscum), a well-known complementary cancer medicine, in zebrafish larvae.
MATERIALS AND METHODS:
Wild-type AB zebrafish embryos at 4 h-post-fertilization were exposed to 5 Gy 9-MeV electron beam irradiation after being treated for 1 h with 4 mMl/L amifostine or 0.2 mg/ml Abnoba Viscum A, F, M, or Q. Primary endpoints were abnormality-free survival and abnormality-free rates among survivors at 5 days-post-fertilization.
RESULTS:
The crude abnormality-free survival rates were 33.7%, 49.0%, 38.8%, 43.9%, 38.1%, and 52.6%, whereas abnormality-free rates among survivors were 36.4%, 49.6%, 37.8%, 45.6%, 52.0%, and 62.8% for the control (with no pharmacologic treatment), amifostine, Abnoba Viscum A, F, M, and Q groups, respectively. Abnormality-free survival rates in the amifostine and Abnoba Viscum Q groups were significantly different from those in the control (p = .040 and .012, respectively), with an odds ratio (OR) of 1.90 [95% confidence interval (CI): 1.03-3.51] and 2.20 (95% CI: 1.19-4.08), respectively. Abnormality-free rates among survivors in the amifostine and Abnoba Viscum M and Q groups were significantly different from those in the control group (p = .048, .042, and <.001, respectively), with an OR of 1.79 (95% CI: 1.00-3.20), 1.82 (95% CI: 1.02-3.26), and 2.98 (1.67-5.33), respectively.
CONCLUSION:
Abnoba Viscum Q has at least a similar radioprotective effect to that of amifostine. Mistletoe extracts have been clinically applied for a long time and their effectiveness and feasibility have been verified. Abnoba Viscum Q might be a new candidate radioprotectant to enhance cancer radiotherapy efficacy.
Author information
Rim CH1, Koun S2, Park HC3, Lee S4, Kim CY4.
1
a Department of Radiation Oncology , Korea University Ansan Hospital , Gyeonggido , Republic of Korea.
2
b Biomedical Research Center Korea University Ansan Hospital , Gyeonggido , Republic of Korea.
3
c Laboratory of Neurodevelopmental Genetics , Korea University Graduate School of Medicine , Seoul , Republic of Korea.
4
d Department of Radiation Oncology , Korea University Anam Hospital , Seoul , Republic of Korea.
편집위원
겨우살이 추출물에서의 방사선 보호제 효과여부를 zebra fish 모델을 이용하여, 연구한 논문으로, 방사선 보호제의 효과 검증을 위한 다양한 in vivo 모델 적용가능성에 대한 흥미로운 논문이하고 사료됨
2019-09-27 15:15:01