글로벌 연구동향
방사선생물학
- 2017년 10월호
[Radiat Oncol J.] Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation.순천향대/ 유병철, 여승구*
- 출처
- Radiat Oncol J.
- 등재일
- 2017 Sep 15
- 저널이슈번호
- doi: 10.3857/roj.2017.00255. [Epub ahead of print]
- 내용
Abstract
Purpose:
The serum carcinoembryonic antigen (CEA) level has been recognized as a prognostic factor in colorectal cancer, and associated with response of rectal cancer to radiotherapy. This study aimed to identify CEA-interacting proteins in colon cancer cells and observe post-irradiation changes in their expression.
Materials and Methods:
CEA expression in colon cancer cells was examined by Western blot analysis. Using an anti-CEA antibody or IgG as a negative control, immunoprecipitation was performed in colon cancer cell lysates. CEA and IgG immunoprecipitates were used for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Proteins identified in the CEA immunoprecipitates but not in the IgG immunoprecipitates were selected as CEA-interacting proteins. After radiation treatment, changes in expression of CEA-interacting proteins were monitored by Western blot analysis.
Results:
CEA expression was higher in SNU-81 cells compared with LoVo cells. The membrane localization of CEA limited the immunoprecipitation results and thus the number of CEA-interacting proteins identified. Only the Ras-related protein Rab-6B and lysozyme C were identified as CEA-interacting proteins in LoVo and SNU-81 cells, respectively. Lysozyme C was detected only in SNU-81, and CEA expression was differently regulated in two cell lines; it was down-regulated in LoVo but up-regulated in SNU-81 in radiation dosage-dependent manner.
Conclusion:
CEA-mediated radiation response appears to vary, depending on the characteristics of individual cancer cells. The lysozyme C and Rab subfamily proteins may play a role in the link between CEA and tumor response to radiation, although further studies are needed to clarify functional roles of the identified proteins.
Author information
Yoo BC1, Yeo SG2.
1Colorectal Cancer Branch, Research Institute, National Cancer Center, Goyang, Korea.2Department of Radiation Oncology, Soonchunhyang University College of Medicine, Soonchunhyang University Hospital, Cheonan, Korea
- 키워드
- Colorectal neoplasms; Lysozyme C; Rab-6B; Radiation; Carcinoembryonic antigen
- 덧글달기
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