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Abstract

The clinical irradiation therapy for cancer could increase the risk of localized wound complications. This study was to evaluate the potential use of a chitosan microparticle-pluronic F127 (CSMP-PF) hydrogel complex containing bioactive molecules, substance P and transforming growth factor-β1, to regeneratively repair skin damaged by local ionizing radiation (IR). The BALB/c/bkl mice were locally irradiated to their limbs with a single 40 Gy dose of Co-60 gamma rays to induce a skin injury. The morphologic characteristics of the chitosan microparticles were analyzed by scanning electron microscopy. The amounts of bioactive molecules taken up and released by the CSMP-PF hydrogel complex were measured. Hematoxylin and eosin staining of IR damaged skin showed acanthosis and hyperkeratosis in the epidermis; and damage to hair follicles/skin appendages and adipose tissue, as well as panniculus carnosus, in the dermis. Injection of the CSMP-PF hydrogel complex into IR damaged skin resulted in skin repair, suggesting that the complex has potential for use in the regenerative repair of IR damaged skin. 

Author information

Park SJ1, Cho W1,2, Kim MS1, Gu BK1, Kang CM1, Khang G3, Kim CH1.​

1Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.2Department of Genetic Engineering, Kyung Hee University, Yongin, Korea.3Department of Polymer-Nano Science and Technology, Polymer Fusion Research Center, Chonbuk National University, Jeonju, Korea.