전북대 / 오필선, 강경원, 이상명*, 정환정*
Abstract
Most recently, severe acute respiratory syndrome coronavirus-2 has triggered a global pandemic without successful therapeutics. The goal of the present study was to define the antiviral effect and therapeutic action of blue light irradiation in SARS-CoV-2-infected cells. Vero cells were infected with SARS-CoV-2 (NCCP43326) or mock inoculum at 50 pfu/well. After blue light irradiation, the inhibitory effect was assessed by qPCR and plaque reduction assay. When Vero cells were irradiated to blue light ranging from 1.6 to 10 J cm-2 , SARS-CoV-2 replication was inhibited by up to 80%. The antiviral effect of blue light irradiation was associated with translation suppression via the phosphorylation of eIF2α by prolonging endoplasmic reticulum (ER) stress. The levels of LC3A/B and Beclin-1, which are key markers of autophagy, and the levels of PERK and PDI for ER stress were highly increased, whereas caspase-3 cleavage was inhibited after blue light irradiation in the later stage of infection. Our data revealed that blue light irradiation exerted antiviral and photo-biogoverning activities by prolonging ER stress and stimulating autophagy progression during viral infection. The findings increase our understanding of how photo-energy acts on viral progression and have implications for use in therapeutic strategies against COVID-19.
Affiliations
Phil-Sun Oh 1 , Kyung Won Kang 2 , Seung Rok Ryu 2 , SeokTae Lim 1 , Myung-Hee Sohn 1 , Sang-Myeong Lee 2 3 , Hwan-Jeong Jeong 1
1 Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Biomedical Research Institute, Jeonbuk National University Medical School and Hospital, Jeonju, Korea.
2 Division of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan, Korea.
3 Laboratory of Veterinary Virology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
편집위원
현재까지의 핵의학 영역의 기술에서 나아가 핵의학과의 융합이 가능한 photo energy 기반의 연구가 기대되고, 이번 코로나 사태와 같이 앞으로도 계속 발생할 가능성이 높은 전염성질환 등의 바이러스 기반 연구의 활성화에 도움이 될 것으로 생각됩니다.
2022-05-04 16:40:21