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갑상선이 제거된 우측의 마우스모델에서 종양의 Tc-99m pertechnetate의 축적이 더 높게 나타남.​

Abstract

We optimized the protocol for thyroid ablation in living mice using radioactive iodine (RAI) and a low-iodine diet (LID). To examine the effect of LID on thyroid ablation, mice were randomly divided into 4 groups: Vehicle, 131I 2.775 MBq, 131I 5.55 MBq, and LID + 131I 2.775 MBq. The LID group was fed a LID for up to 7 days and then mice in the 131I 2.775, 131I 5.55, and LID + 131I 2.775 MBq groups were intravenously administrated with 131I, respectively. Scintigraphy imaging with 99mTc pertechnetate was performed once in 2 weeks for 4 weeks. After establishment of athyroid mice, control or athyroid mice were injected with human anaplastic thyroid cancer cells co-expressing sodium iodine symporter and enhanced firefly luciferase (ARO/NF) to evaluate RAI uptake. Scintigraphy imaging with 99mTc pertechnetate was performed with ARO/NF tumor-bearing mice. Scintigraphy imaging showed decreased thyroid uptake in the LID + 131I 2.775 MBq group compared to other groups. Scintigraphy images showed that tumor uptake was statically higher in athyroid mice than in control mice. These data suggest that these optimized conditions for thyroid ablation could be helpful to establish an in vivo mouse model.

Author information

Oh JM1, Lee HW1, Kalimuthu S1, Gangadaran P1, Baek SH1, Han MH2, Hong CM1, Jeong SY1, Lee SW1, Lee J1,3, Ahn BC4.

1 Department of Nuclear Medicine, Kyungpook National University and Hospital, Daegu, South Korea.

2 Department of Pathology, Kyungpook National University and Hospital, Daegu, South Korea.

3 Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), 80 Chembok-ro, Dong-gu, Daegu, South Korea.

4 Department of Nuclear Medicine, Kyungpook National University and Hospital, Daegu, South Korea. abc2000@knu.ac.kr.