방사선의학 웹진

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타닉엑시드(tannic acid)가 코팅되어 있는 금나노입자에 수백개 이상의 방사성동위원소를 표지하고 추가적으로 금껍질을 형성시켜 핵의학영상에 응용할 수 있는 새로운 조영제 개발기술을 개발하였음​

본 연구에서 개발한 핵의학영상 조영제를 이용하여 수지상세포 표지를 한 후 이들이 근처 림프절로 이동되는 현상을 핵의학 영상기술로로 성공적으로 영상화함​

Abstract

The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization of dendritic cell (DC) migration is one of the essential factors for effective DC-based immunotherapy. Here, we used a novel and efficient synthesis approach to develop radioiodine-124-labeled tannic acid gold core-shell nanoparticles (124I-TA-Au@AuNPs) for DC labeling and in vivo tracking of their migration using positron emission tomography (PET). 124I-TA-Au@AuNPs were produced within 40 min in high yield via straightforward tannic acid-mediated radiolabeling chemistry and incorporation of Au shell, which resulted in high radio-sensitivity and excellent chemical stability of nanoparticles in DCs and living mice. 124I-TA-Au@AuNPs demonstrated good DC labeling efficiency and did not affect cell biological functions, including proliferation and phenotype marker expression. Importantly, 124I-TA-Au@AuNPs in an extremely low amount (0.1 mg/kg) were successfully applied to track the migration of DCs to lymphoid organs (draining lymph nodes) in mice.

Author information

Lee SB, Lee SW, Jeong SY, Yoon G, Cho SJ1, Kim SK, Lee IK, Ahn BC, Lee J1, Jeon YH.

1 Daegu-Gyeongbuk Medical Innovation Foundation , Daegu 41061, Korea.