글로벌 연구동향
핵의학
- 2017년 02월호
[Biomaterials. ] Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis.서울의대, 고려대 / 오현정, 이동수*, 정석*, 황도원*
- 출처
- Biomaterials.
- 등재일
- 2017 Jan
- 저널이슈번호
- 112:82-94. doi: 10.1016/j.biomaterials.2016.10.006. Epub 2016 Oct 5.
- 내용
Abstract
The effective role of exosome delivering neurogenic microRNA (miRNA) enables to induce efficient differentiation process during neurogenesis. The microfludic system capable of visualizing the exosomal behavior such as secretion, migration, and uptake of individual exosomes can be used as a robust technique to understand the exosome-mediated change of cellular behavior. Here, we developed the exosome-tracing microfluidic system to visualize exosomal transport carrying the neurogenic miRNA from leading to neighboring cells, and found a new mode of exosome-mediated cell-non-autonomous neurogenesis. The miR-193a facilitated neurogenesis in F11 cells by blocking proliferation-related target genes. In addition to time-lapse live-cell imaging using microfluidics visualized the convective transport of exosomes from differentiated to undifferentiated cells. Individual exosomes containing miR-193a from differentiated donor cells were taken up by undifferentiated cells to lead them to neurogenesis. Induction of anti-miR-193a was sufficient to block neurogenesis in F11 cells. Inhibition of the exosomal production by manumycin-A and treatment of anti-miR-193a in the differentiated donor cells failed to induce neurogenesis in undifferentiated recipient cells. These findings indicate that exosomes of neural progenitors and neurogenic miRNA within these exosomes propagate cell-non-autonomous differentiation to neighboring progenitors, to delineate the roles of exosome mediating neurogenesis of population of homologous neural progenitor cells.
Author information
Oh HJ1, Shin Y2, Chung S3, Hwang DW4, Lee DS5.
1Department of Nuclear Medicine, Seoul National University College of Medicine, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, and College of Medicine or College of Pharmacy, Seoul National University, Republic of Korea; School of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
2School of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
3School of Mechanical Engineering, Korea University, Seoul, Republic of Korea. Electronic address: sidchung@gmail.com.
4Department of Nuclear Medicine, Seoul National University College of Medicine, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, and College of Medicine or College of Pharmacy, Seoul National University, Republic of Korea. Electronic address: hdw6592@hanmail.net.
5Department of Nuclear Medicine, Seoul National University College of Medicine, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, and College of Medicine or College of Pharmacy, Seoul National University, Republic of Korea. Electronic address: dsl@plaza.snu.ac.kr.
- 키워드
- Cell-non-autonomous; Exosome imaging; MiR-193a; Microfluidic platform; Neurogenesis
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