분자영상 및 방사화학

  • [Sci Rep.] New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging.

    Mayo Clinic / Peng KW*

  • 출처
    Sci Rep.
  • 등재일
    2018 Sep 21
  • 저널이슈번호
    8(1):14209. doi: 10.1038/s41598-018-32442-x.
  • 내용

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    Abstract
    Fibrogenesis is the underlying mechanism of wound healing and repair. Animal models that enable longitudinal monitoring of fibrogenesis are needed to improve traditional tissue analysis post-mortem. Here, we generated transgenic reporter rats expressing the sodium iodide symporter (NIS) driven by the rat collagen type-1 alpha-1 (Col1α1) promoter and demonstrated that fibrogenesis can be visualized over time using SPECT or PET imaging following activation of NIS expression by rotator cuff (RC) injury. Radiotracer uptake was first detected in and around the injury site day 3 following surgery, increasing through day 7-14, and declining by day 21, revealing for the first time, the kinetics of Col1α1 promoter activity in situ. Differences in the intensity and duration of NIS expression/collagen promoter activation between individual RC injured Col1α1-hNIS rats were evident. Dexamethasone treatment delayed time to peak NIS signals, showing that modulation of fibrogenesis by a steroid can be imaged with exquisite sensitivity and resolution in living animals. NIS reporter rats would facilitate studies in physiological wound repair and pathological processes such as fibrosis and the development of anti-fibrotic drugs.

     


    Author information

    Brunton B1, Suksanpaisan L2, Li H2, Liu Q3, Yu Y3, Vrieze A3, Zhang L1, Jenks N1, Jiang H4, DeGrado TR4, Zhao C3, Russell SJ1, Peng KW5.
    1
    Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA.
    2
    Imanis Life Sciences, Rochester, MN, USA.
    3
    Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA.
    4
    Department of Radiology, Mayo Clinic, Rochester, MN, USA.
    5
    Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA. peng.kah@mayo.edu.

  • 편집위원

    NIS reporter gene을 이용하여 fibrogenesis 형성과정을 형질전환 rat을 제작하여 살아있는 개체내에서 fibrogenesis 과정을 실시간으로 모니터링한 결과를 처음으로 보여주었음.

    2018-10-23 12:26:54

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