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Abstract

PURPOSE: 

The aim of this study was to explore the potential for radiation-specific signaling of various LDIR-induced effects in human B-lymphoblast IM-9 cells.

MATERIALS AND METHODS: 

Human lymphoblast IM-9 cells were exposed to ionization radiation at 0.1 and 2 Gy using a 137Cs γ-irradiator at a dose rate of 0.8 Gy/min. Cell viability and DNA fragmentation was determined using MTT assay and TUNEL assay at 24 hr after irradiation. Profiling of protein phosphorylation by radiation was identified using a phospho-antibody array at 4 hr after irradiation and Dataset of the profiling was analyzed by IPA.

RESULTS: 

Cell survival and apoptotic signaling was not affected by 0.1 Gy of radiation, whereas 2 Gy induced cellular damage. The analysis of low-dose ionizing radiation (LDIR) or high-dose ionizing radiation (HDIR)-specific responses by IPA generated different results. Various cell maintenance functions were only apparent following the analysis of increased protein phosphorylation by LDIR, whereas several cancer formation- and development-related functions were only detected following the analysis of increased protein phosphorylation by HDIR Conclusions: The LDIR-induced protein phosphorylation patterns might be involved in various cell survival responses or cellular maintenance functions, which provide important insight into our understanding of the different effects of LDIR and HDIR.​ 

Author information

Eom HS1, Park HS1, You GE1, Kim JY1, Nam SY1

1a Low-dose Radiation Research Team, Radiation Health Institute, Korea Hydro & Nuclear Power Co. LTD. , Seoul , Korea.