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  • [Cancer Res.] [18F](2S,4R)4-Fluoroglutamine PET Detects Glutamine Pool Size Changes in Triple-Negative Breast Cancer in Response to Glutaminase Inhibition

    University of Pennsylvania / Rong Zhou*

  • 출처
    Cancer Res.
  • 등재일
    2017 Mar 15
  • 저널이슈번호
    77(6):1476-1484. doi: 10.1158/0008-5472.CAN-16-1945. Epub 2017 Feb 15.
  • 내용

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    Abstract

    Glutaminolysis is a metabolic pathway adapted by many aggressive cancers, including triple-negative breast cancers (TNBC), to utilize glutamine for survival and growth. In this study, we examined the utility of [18F](2S,4R)4-fluoroglutamine ([18F]4F-Gln) PET to measure tumor cellular glutamine pool size, whose change might reveal the pharmacodynamic (PD) effect of drugs targeting this cancer-specific metabolic pathway. High glutaminase (GLS) activity in TNBC tumors resulted in low cellular glutamine pool size assayed via high-resolution 1H magnetic resonance spectroscopy (MRS). GLS inhibition significantly increased glutamine pool size in TNBC tumors. MCF-7 tumors, with inherently low GLS activity compared with TNBC, displayed a larger baseline glutamine pool size that did not change as much in response to GLS inhibition. The tumor-to-blood-activity ratios (T/B) obtained from [18F]4F-Gln PET images matched the distinct glutamine pool sizes of both tumor models at baseline. After a short course of GLS inhibitor treatment, the T/B values increased significantly in TNBC, but did not change in MCF-7 tumors. Across both tumor types and after GLS inhibitor or vehicle treatment, we observed a strong positive correlation between T/B values and tumor glutamine pool size measured using MRS (r2 = 0.71). In conclusion, [18F]4F-Gln PET tracked cellular glutamine pool size in breast cancers with differential GLS activity and detected increases in cellular glutamine pool size induced by GLS inhibitors. This study accomplished the first necessary step toward validating [18F]4F-Gln PET as a PD marker for GLS-targeting drugs.

     

    Author information

    Zhou R1, Pantel AR2, Li S2, Lieberman BP2, Ploessl K2, Choi H2, Blankemeyer E2, Lee H2, Kung HF2, Mach RH2, Mankoff DA1.

    Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania. rongzhou@upenn.edu david.mankoff@uphs.upenn.edu.

    Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania.

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