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Abstract
A quintinite nanoplate (64Cu-QT-NP) isomorphically substituted with 64Cu, as the positron emission tomography (PET) imaging material, was prepared via two-step processes. A 64Cu labeling efficiency of 99% was realized, for the first time, by immobilizing the 64Cu radioisotope directly in the octahedral site of the 2-dimensional (2D) quintinite lattice. Furthermore, the 64Cu labeling stability of 64Cu-QT-NPs was also achieved to be more than ∼99% in various solutions such as saline, phosphate-buffered saline (PBS), and other biological media (mouse and human serums). In an in vivo xenograft mouse model, the passive targeting behavior of 64Cu-QT-NPs into tumor tissue based on the enhanced permeability and retention (EPR) effect was also demonstrated by parenteral administration, and successfully visualized using a PET scanner. For enhancing the tumor tissue selectivity, bovine serum albumin (BSA) was coated on 64Cu-QT-NPs to form 64Cu-QT-NPs/BSA, resulting in better colloidal stability and longer blood circulation time, which was eventually evidenced by the 2-fold higher tumor uptake rate when intravenousely injected in an animal model. It is, therefore, concluded that the present 64Cu-QT-NPs/BSA with tumor tissue selectivity could be an advanced nano-device for radio-imaging and diagnosis as well.

Affiliations

Sairan Eom  1   2 , Min Hwan Kim  2 , Ranji Yoo  2 , Goeun Choi  3   4   5 , Joo Hyun Kang  2 , Yong Jin Lee  2 , Jin-Ho Choy  3   6   7
1 Center for Intelligent Nano-Bio Materials (CINBM), Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.
2 Division of Applied-RI, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea.
3 Intelligent Nanohybrid Materials Laboratory (INML), Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, Republic of Korea. jhchoy@dankook.ac.kr.
4 College of Science and Technology, Dankook University, Cheonan 31116, Republic of Korea.
5 Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea.
6 Department of Pre-medical Course, College of Medicine, Dankook University, Cheonan 31116, Republic of Korea.
7 International Research Frontier Initiative (IRFI), Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan.