서울의대 / 이석용, 오호림, 윤혜원*
Abstract
Rationale: [18F]fluorodeoxyglucose-positron emission tomography ([18F]FDG-PET) has been widely used as an imaging technique to measure interscapular brown adipose tissue (iBAT) activity. However, it is challenging to obtain iBAT-specific images using [18F]FDG-PET because increased uptake of [18F]FDG is observed in tumors, muscle, and inflamed tissues. Uncoupling protein 1 (UCP1) in the mitochondrial membrane, a well-known molecular marker of BAT, has been proposed as a useful BAT imaging marker. Recently, the UCP1 ThermoMouse was developed as a reporter mouse for monitoring UCP1 expression and investigating BAT activation. In addition, Translocator protein-18 kDa (TSPO) located in the outer mitochondrial membrane is also overexpressed in BAT, suggesting that TSPO-targeting PET has potential for iBAT imaging. However, there are no studies monitoring BAT using TSPO-targeting PET probes in the UCP1 ThermoMouse. Moreover, the non-invasive Cerenkov luminescence imaging (CLI) using Cerenkov radiation from the PET probe has been proposed as an alternative option for PET as it is less expensive and user-friendly. Therefore, we selected [18F]fm-PBR28-d2 as a TSPO-targeting PET probe for iBAT imaging to evaluate the usefulness of CLI in the UCP1 ThermoMouse.
Methods: UCP1 ThermoMouse was used to monitor UCP1 expression. Western blotting and immunohistochemistry were performed to measure the level of protein expression. [18F]fm-PBR28-d2 and [18F]FDG were used as radioactive probes for iBAT imaging. PET images were acquired with SimPET, and optical images were acquired with IVIS 100.
Results: UCP1 ThermoMouse showed that UCP1 and TSPO expressions were correlated in iBAT. In both PET and CLI, the TSPO-targeting probe [18F]fm-PBR28-d2 was superior to [18F]FDG for acquiring iBAT images. The high molar activity of the probe was essential for CLI and PET imaging. We tested the feasibility of TSPO-targeting probe under cold exposure by imaging with TSPO-PET/CLI. Both signals of iBAT were clearly increased after cold stimulation. Under prolonged isoflurane anesthesia, TSPO-targeting images showed higher signals from iBAT in the short-term than in long-term groups.
Conclusion: We demonstrated that TSPO-PET/CLI reflected UCP1 expression in iBAT imaging better than [18F]FDG-PET/CLI under the various conditions. Considering convenience and cost, TSPO-CLI could be used as an alternative TSPO-PET technique for iBAT imaging.
.
Affiliations
Seok-Yong Lee1,2,3#, Ho Rim Oh1,2,3#, Young-Hwa Kim1,3, Sung-Hwan Bae1,3, Yongseok Lee4, Yun-Sang Lee1,4,5, Byung Chul Lee6,7, Gi Jeong Cheon1,3, Keon Wook Kang1,2,3, Hyewon Youn1,3,4
1. Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
2. Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Republic of Korea
3. Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
4. Cancer Imaging Center, Seoul National University Hospital, Seoul, Republic of Korea
5. Radiation Medicine Research Institute, Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea
6. Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea
7. Center for Nanomolecular Imaging and Innovative Drug Development, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
# These authors equally contributed to this research.
편집위원
Cerenkov luminescence는 Cerenkov radiation을 이용하여 bioluminescence같은광학영상을 얻는 영상 기술이다. 따라서 F-18 방사성표지자를 이용하면 PET 영상뿐만 아니라 Cerenkov luminescence 영상까지 얻을 수 있다. Brown fat으로 알려진 interscapular brown adipose tissue (iBAT)는 [F-18]FDG PET으로도 일부 영상이 가능하지만, [F-18]FDG는 원래 종양 영상 목적의 방사성의약품이다. 따라서, 최적의 BAT 영상을 위해 이 논문은 Translocator protein-18 kDa (TSPO)를 표적으로 하는 F-18 바이오마커인 [F-18]fm-PBR28-d2를 이용하여 TSPO-PET 및 TSPO-CLI iBAT 영상 결과를 보여주고 있다.
2022-10-06 10:10:28
편집위원
Cerenkov luminescence는 Cerenkov radiation을 이용하여 bioluminescence같은광학영상을 얻는 영상 기술이다. 따라서 F-18 방사성표지자를 이용하면 PET 영상뿐만 아니라 Cerenkov luminescence 영상까지 얻을 수 있다. Brown fat으로 알려진 interscapular brown adipose tissue (iBAT)는 [F-18]FDG PET으로도 일부 영상이 가능하지만, [F-18]FDG는 원래 종양 영상 목적의 방사성의약품이다. 따라서, 최적의 BAT 영상을 위해 이 논문은 Translocator protein-18 kDa (TSPO)를 표적으로 하는 F-18 바이오마커인 [F-18]fm-PBR28-d2를 이용하여 TSPO-PET 및 TSPO-CLI iBAT 영상 결과를 보여주고 있다.
2022-12-06 15:26:15