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Abstract
Purpose: Plastics are used commonly in the world because of its convenience and cost-effectiveness. Microplastics, an environmental threat and human health risk, are widely detected in food, and consequently ingested. However degraded plastics are found everywhere, which cause environmental threat and human health risk. Therefore, real-time monitoring of orally administered microplastics is tremendously important to trace them in the body. Methods: In this study, to visualize their absorption path, we labeled polystyrene with [64Cu]Cu-DOTA. We prepared radiolabeled polystyrene with 64Cu after, [64Cu]Cu-DOTA-polystyrene was then orally administered to mice and evaluate its transit and absorption in mice using PET imaging. The absorption path and distribution of [64Cu]Cu-DOTA-polystyrene were determined using positron emission tomography (PET) over 48 h. Ex vivo tissue-radio thin-layer chromatography (Ex vivo-radioTLC) was used to demonstrate the existence of [64Cu]Cu-DOTA-polystyrene in tissue. Results: PET images demonstrated that [64Cu]Cu-DOTA-polystyrene began to transit to the intestine within 1 h. [64Cu]Cu-DOTA-polystyrene accumulation in the liver was also observed. Biodistribution of [64Cu]Cu-DOTA-polystyrene confirmed the observed distribution of [64Cu]Cu-DOTA-polystyrene from PET images. Ex vivo-radioTLC was used to demonstrate that the detected gamma rays originated from [64Cu]Cu-DOTA-polystyrene. Conclusion: This study provided evidence of microplastic accumulation and existence in tissue by using PET imaging, and cross confirmed by ex vivo-radioTLC. The information provided may be used as the basis for future studies on the toxicity of microplastics.

 64Cu-DOTA-폴리스티렌 체내 흡수 경로 PET 영상

Affiliations

Changkeun Im  1 , Hyeongi Kim  1 , Javeria Zaheer  1 , Jung Young Kim  1 , Yong Jin Lee  2 , Choong Mo Kang  2 , Jin Su Kim  2
1 Korea Institute of Radiological & Medical Sciences.
2 Korea Institute of Radiological and Medical Sciences, Korea, Republic of.