이화의대, (주)유머스트알앤디, 건국대 / 백수지, 김지수, 문병석*, 김범산*, 이강파*
Abstract
Sarcopenia- or cachexia-related muscle atrophy is due to imbalanced energy metabolism and oxidative stress-induced muscle dysfunction. Monoterpenes play biological and pharmacological reactive oxygen species (ROS) scavenging roles. Hence, we explored the effects of camphene, a bicyclic monoterpene, on skeletal muscle atrophy in vitro and in vivo. We treated L6 myoblast cells with camphene and then examined the ROS-related oxidative stress using Mito TrackerTM Red FM and anti-8-oxoguanine antibody staining. To investigate lipid metabolism, we performed real-time polymerase chain reactions, holotomographic microscopy, and respiratory gas analysis. Rat muscle atrophy in in vivo models was observed using 18F-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography and immunocytochemistry. Camphene reversed the aberrant cell size and muscle morphology of L6 myoblasts under starvation and in in vivo models. Camphene also attenuated E3 ubiquitin ligase muscle RING-finger protein-1, mitochondrial fission, and 8-oxoguanine nuclear expression in starved myotubes and hydrogen peroxide (H2O2)-treated cells. Moreover, camphene significantly regulated lipid metabolism in H2O2-treated cells and in vivo models. These findings suggest that camphene may potentially affect skeletal muscle atrophy by regulating oxidative stress and lipid metabolism.
Affiliations
Suji Baek 1 , Jisu Kim 2 , Byung Seok Moon 3 , Sun Mi Park 3 , Da Eun Jung 3 , Seo Young Kang 3 , Sang Ju Lee 4 , Seung Jun Oh 4 , Seung Hae Kwon 5 , Myung Hee Nam 5 , Hye Ok Kim 3 , Hai Jeon Yoon 3 , Bom Sahn Kim 3 , Kang Pa Lee 1
1 Research and Development Center, UMUST R&D Corporation, Seoul 05029, Korea.
2 Physical Activity and Performance Institute, Konkuk University, Seoul 05029, Korea.
3 Department of Nuclear Medicine, Ewha Womans University College of Medicine, Seoul 07804, Korea.
4 Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.
5 Seoul Center, Korean Basic Science Institute, Seoul 02841, Korea.