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  • [Exp Cell Res.] Extracellular vesicles derived from macrophage promote angiogenesis In vitro and accelerate new vasculature formation In vivo

    [Exp Cell Res.] Extracellular vesicles derived from macrophage promote angiogenesis In vitro and accelerate new vasculature formation In vivo

    경북의대 / Prakash, 안병철*

  • 출처
    Exp Cell Res.
  • 등재일
    2020 Sep 15
  • 저널이슈번호
    394(2):112146. doi: 10.1016/j.yexcr.2020.112146. Epub 2020 Jun 17.
  • 내용

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    Abstract
    Background: Ischemia is the partial or complete blockage of blood supply to tissues. Extracellular vesicles (EVs) are emerging as a therapeutic tool for ischemic diseases. Most EV-based ischemia therapies are based on various stem cells. Here, we propose an alternative cell source for the isolation of pro-angiogenic EVs.

    Methods: EVs were isolated from a mouse macrophage cell line (Raw 264.7). The characteristic features of the macrophage-derived EVs (MAC-EVs) were assessed using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting (WB) analysis. WB and qRT-PCR were performed to identify the pro-angiogenic VEGF and Wnt3a proteins and microRNAs (miR-210, miR-126, and miR-130a) in the MAC-EVs. In vitro and in vivo Matrigel plug assays were performed to investigate the capacity of the MAC-EVs for tube (blood vessel-like) formation and new blood vessel formation and assessed by histology.

    Results: The MAC-EVs was positive for ALIX and negative for calnexin, with a round shape and an average size of 189 ± 65.1 nm. WB and qRT-PCR results revealed that VEGF, Wnt3a and miR-130a were more abundant in the MAC-EVs than cells. MAC-EVs treatment resulted in increased endothelial cellular proliferation, migration, and tube formation in vitro. In vivo assay results revealed that MAC-EVs increased the formation of new and larger blood vessels in the Matrigel plug of mice compared to the formation in the control group.

    Conclusion: Our results suggest that MAC-EVs have the potential to induce angiogenesis in vitro and in vivo, could serve as a pro-angiogenic alternative for ischemic diseases.

     



    Affiliations

    Prakash Gangadaran  1 , Ramya Lakshmi Rajendran  2 , Ji Min Oh  2 , Chae Moon Hong  3 , Shin Young Jeong  4 , Sang-Woo Lee  4 , Jaetae Lee  4 , Byeong-Cheol Ahn  5
    1 Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
    2 Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
    3 Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
    4 Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
    5 Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea. Electronic address: abc2000@knu.ac.kr.

  • 키워드
    Angiogenesis; Extracellular vesicle; Macrophage cell; miRNA.
  • 연구소개
    허혈성 질환은 심장, 뇌를 포함한 다양한 장기에 혈류 공급 부족으로 발생하며 혈관재생이 치료에 도움이 됩니다. 이 연구는 허혈성 질환에 대식세포 유래 세포외 소포의 혈관신생효과를 세포 및 동물 실험으로 확인한 연구입니다. 대식세포 유래 소포외 소포는 proangiogenic factors를 포함하고 있으며, 내피세포의 분열, 이동 및 tube 형성능을 높이며, 동물실험에서도 혈관형성능이 있음을 확인하였습니다. 향후 대식세포 유래 세포외 소포가 허혈성 질환의 치료제로 개발될 수 있을 것으로 생각됩니다. 따라서, 이 연구는 세포외 소포 및 세포외 소포 유사체 관련 연구자들에게 도움이 될 만한 좋은 정보라 생각합니다.
  • 편집위원

    대식세포에서 분리된 세포외세포의 혈관신생 능력을 세포 실험 및 동물실험을 통해 보여준 연구임. 혈관신생 관련 연구자 및 세포외 소포 관련 연구자에게 관심을 유도할 연구로 생각됨.

    2020-11-10 09:46:11

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