글로벌 연구동향
분자영상 및 방사화학
- 2020년 10월호
[Theranostics.] A novel nucleolin-binding peptide for Cancer Theranostics광주과학기술원 / 김재현, 남정숙*, 김재일*
- 출처
- Theranostics.
- 등재일
- 2020 Jul 14
- 저널이슈번호
- 10(20):9153-9171. doi: 10.7150/thno.43502. eCollection 2020.
- 내용
Abstract
Background: Cancer-specific ligands have been of great interest as pharmaceutical carriers due to the potential for site-specific delivery. In particular, cancer-specific peptides have many advantages over nanoparticles and antibodies, including high biocompatibility, low immunogenicity, and the formation of nontoxic metabolites. The goal of the present study was the development of a novel cancer-specific ligand. Methods: Cancer-specific peptide ligands were screened using a one-bead-one-compound (OBOC) combinatorial method combined with a multiple-antigen-peptide (MAP) synthesis method. The specificity of the peptide ligands toward cancer cells was tested in vitro using a whole-cell binding assay, flow cytometry, and fluorescence confocal microscopy. The tissue distribution profile and therapeutic efficacy of a paclitaxel (PTX)-conjugated peptide ligand was assessed in vivo using xenograft mouse models. Results: We discovered that AGM-330 specifically bound to cancer cells in vitro and in vivo. Treatment with PTX-conjugated AGM-330 dramatically inhibited cancer cell growth in vitro and in vivo compared to treatment with PTX alone. The results of pull-down assay and LC-MS/MS analyses showed that membrane nucleolin (NCL) was the target protein of AGM-330. Although NCL is known as a nuclear protein, we observed that it was overexpressed on the membranes of cancer cells. In particular, membrane NCL neutralization inhibited growth in cancer cells in vitro. Conclusions: In summary, our findings indicated that NCL-targeting AGM-330 has great potential for use in cancer diagnosis and targeted drug delivery in cancer therapy.Affiliations
Jae-Hyun Kim 1 , Chanhyung Bae 2 , Min-Jung Kim 1 , In-Hye Song 1 , Jae-Ha Ryu 3 , Jang-Hyun Choi 1 , Choong-Jae Lee 1 , Jeong-Seok Nam 1 , Jae Il Kim 1 3
1 School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.
2 Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
3 Pilot Plant, Anygen, Gwangju, Technopark, 333 Cheomdankwagi-ro, Buk-gu, Gwangju, 61008, Republic of Korea.
- 키워드
- multiple-antigen-peptide; nucleolin; one-bead-one-compound; paclitaxel; peptide.
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