글로벌 연구동향
분자영상 및 방사화학
- 2019년 08월호
[Bioconjug Chem.] PLGA Microspheres Coated with Cancer Cell-Derived Vesicles for Improved Internalization into Antigen-Presenting Cells and Immune Stimulation.건국대 / 정혜선, 목혜정*
- 출처
- Bioconjug Chem.
- 등재일
- 2019 Jun 19
- 저널이슈번호
- 30(6):1690-1701. doi: 10.1021/acs.bioconjchem.9b00240. Epub 2019 May 8.
- 내용
Abstract
Microspheres (MS; 1-3 μm) with different degrees of surface roughness were prepared to assess the effects of surface topology on internalization into antigen-presenting cells (APCs; macrophages and dendritic cells). In this study, we demonstrated that the intracellular uptake of MS is readily enhanced by surface modification with nanoparticles or cancer cell-derived vesicles (VE) to modulate their surface topology. MS coated with nanovesicles (MS-VE) with high surface roughness was more successfully and efficiently engulfed by APCs, compared with bare MS and those with low surface roughness. Incorporated MPLA within MS-VEs (M/MS-VE) triggered greatly elevated release of immune stimulating cytokines, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), from macrophages and dendritic cells, compared to free MPLA. Taken together, this MS-VE could serve as a platform system for the delivery of immune stimulators and antigens to APCs with negligible toxicity.
Author informationJung H1, Jang HE1, Kang YY1, Song J1, Mok H1.
1
Department of Bioscience and Biotechnology , Konkuk University , Seoul 05029 , Republic of Korea.
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