서울대 / 조아라, 박승범*
Abstract
Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.
Author information
Jo A, Sung J, Lee S1, Nam H, Lee HW, Park J2, Kim HM, Kim E, Park SB.
1
Center for Neuro-Medicine, Brain Science Institute , Korea Institute of Science and Technology , Seoul , 02792 , Korea.
2
Center for Systems Biology, Massachusetts General Hospital , Harvard Medical School , Boston , Massachusetts 02114 , United States.