중앙대 / 한의영, 홍진기*
Abstract
In this study, we established the structure of a multilayer nanofilm that more efficiently encapsulates basic fibroblast growth factor (bFGF). First, a positively charged layer material was selected from biocompatible polymers such as collagen (Col), poly(beta-amino ester) (Poly2), and chitosan (Chi), while considering the film thickness. We then investigated the change in bFGF encapsulation efficiency when the multilayer structure was changed from a tetralayer to a trilayer. As a result, we obtained a highly improved bFGF encapsulation efficiency in the nanofilm using a positively charged layer formed by a blend of Col and Poly2 and a negatively charged poly(acrylic acid) (PAA) layer within a trilayered structure. In particular, we found that a significant amount of adsorbed bFGF was desorbed again during the film fabrication process of a tetralayered nanofilm. In the conventional nanofilm, bFGF was regarded as a polycation and formed a multilayer nanofilm that was composed of a tetralayered structure and was represented as (polycation/polyanion/bFGF/polyanion) n where n = number of repeated tetralayers. Here, we suggested that bFGF should not be considered a polycation, rather it should be considered as a small quantity of molecule that exists between the polyanion and polycation layers. In this case, the nanofilm is composed of repeating units of (polycation/polyanion/bFGF/polycation/polyanion), because the amount of adsorbed bFGF is considerably lower than that of other building blocks.
Author information
Han U1, Hong J1.
1
School of Chemical Engineering and Material Science , Chung-Ang University , 84, Heukseok-ro, Dongjak-gu , Seoul 06974 , Republic of Korea.
편집위원
Basic fibroblast growth factor(bFGF)는 많은 연구에 사용되고 있는 조직 및 줄기세포 재생 등에 관여하는 물질이지만, 불안정한 구조를 갖고 있어서 사용에 어려움이 있다. 이러한 배경 속에서 이 논문은 bFGF를 목표로 하는 부위에 안정적이고 정확하게 운반 및 방출시키기 위한 방법으로써 생체 적합 고분자를 이용한 다층 나노필름 캡슐화(multilayer nanofilm encapsulation) 연구를 보여주고 있다. 이는 성장인자 등 신호전달물질의 정확한 전달을 위한 향상된 플랫폼을 제시하고 있다.
2018-04-17 11:08:58