분자영상 및 방사화학

본문글자크기
  • [Biomaterials.] Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT.

    한양대 / 박현희, 고성호*

  • 출처
    Biomaterials.
  • 등재일
    2018 Feb
  • 저널이슈번호
    155:80-91. doi: 10.1016/j.biomaterials.2017.11.009. Epub 2017 Nov 14.
  • 내용

    바로가기  >

    Abstract
    In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.

     


    Author information

    Park HH1, Lee KY1, Park DW2, Choi NY1, Lee YJ1, Son JW3, Kim S4, Moon C5, Kim HW6, Rhyu IJ6, Koh SH7.
    1
    Department of Neurology, Hanyang University Guri Hospital, 153, Gyeongchun-ro, Guri-si, Gyeonggi-do 11923, South Korea.
    2
    Department of Radiology, Hanyang University Guri Hospital, 153, Gyeongchun-ro, Guri-si, Gyeonggi-do 11923, South Korea.
    3
    Department of Translational Medicine, Hanyang University Graduate School of Biomedical Science & Engineering, Seoul, 04763, South Korea.
    4
    Teloid Inc., 920 Westholme Ave, Los Angeles (City), CA 90024, USA.
    5
    Department of Neuroscience, GemVax & KAEL Co., Ltd., Seoul, South Korea.
    6
    Brain Korea 21 PLUS, KU Medical Science Center for Convergent Translational Research, 73 Inchonro, Seongbuk-gu, Seoul, 136-705, South Korea; Department of Anatomy, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul, 136-705, South Korea.
    7
    Department of Neurology, Hanyang University Guri Hospital, 153, Gyeongchun-ro, Guri-si, Gyeonggi-do 11923, South Korea; Department of Translational Medicine, Hanyang University Graduate School of Biomedical Science & Engineering, Seoul, 04763, South Korea. Electronic address: ksh213@hanyang.ac.kr.

  • 키워드
    MRI; Mesenchymal stem cells; Neural stem cells; Peptide; Tracking; hTERT
  • 덧글달기
    덧글달기
       IP : 3.89.116.152

    등록