글로벌 연구동향
방사선생물학
- 2020년 11월호
[Korean J Physiol Pharmacol.] Delphinidin enhances radio-therapeutic effects via autophagy induction and JNK/MAPK pathway activation in non-small cell lung cancer건양대 / 강성희, 강보선*
- 출처
- Korean J Physiol Pharmacol.
- 등재일
- 2020 Sep 1
- 저널이슈번호
- 24(5):413-422. doi: 10.4196/kjpp.2020.24.5.413.
- 내용
Abstract
Delphinidin is a major anthocyanidin compound found in various vegetables and fruits. It has anti-oxidant, anti-inflammatory, and various other biological activities. In this study we demonstrated the anti-cancer activity of delphinidin, which was related to autophagy, in radiation-exposed non-small cell lung cancer (NSCLC). Radiosensitising effects were assessed in vitro by treating cells with a subcytotoxic dose of delphinidin (5 μM) before exposure to γ-ionising radiation (IR). We found that treatment with delphinidin or IR induced NSCLC cell death in vitro; however the combination of delphinidin pre-treatment and IR was more effective than either agent alone, yielding a radiation enhancement ratio of 1.54 at the 50% lethal dose. Moreover, combined treatment with delphinidin and IR, enhanced apoptotic cell death, suppressed the mTOR pathway, and activated the JNK/MAPK pathway. Delphinidin inhibited the phosphorylation of PI3K, AKT, and mTOR, and increased the expression of autophagy-induced cell death associated-protein in radiation-exposed NSCLC cells. In addition, JNK phosphorylation was upregulated by delphinidin pre-treatment in radiation-exposed NSCLC cells. Collectively, these results show that delphinidin acts as a radiation-sensitizing agent through autophagy induction and JNK/MAPK pathway activation, thus enhancing apoptotic cell death in NSCLC cells.Affiliations
Seong Hee Kang 1 2 , Dong-Ho Bak 1 , Byung Yeoup Chung 1 , Hyoung-Woo Bai 1 , Bo Sun Kang 2
1 Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, Korea.
2 Department of Medical Science, Konyang University, Daejeon 35365, Korea.
- 키워드
- Autophagy; Delphinidin; Non-small-cell lung carcinoma; Radiation-sensitizing agent.
- 덧글달기
- 이전글 [Br J Cancer.] CB11, a novel purine-based PPARɣ ligand, overcomes radio-resistance by regulating ATM signalling and EMT in human non-small-cell lung cancer cells
- 다음글 [Int J Radiat Oncol Biol Phys.] Combined NK Cell Therapy and Radiation Therapy Exhibit Long-Term Therapeutic and Antimetastatic Effects in a Human Triple Negative Breast Cancer Model