방사선생물학

본문글자크기
  • [Exp Dermatol.] Integrin αvβ3-Akt signalling plays a role in radioresistance of melanoma.

    KIRAMS / 임현택, 이재연*

  • 출처
    Exp Dermatol.
  • 등재일
    2020 Apr 16. doi: 10.1111/exd.14102. [Epub ahead o
  • 저널이슈번호
  • 내용

    바로가기  >

    Abstract
    Melanoma is a deadly type of skin cancer that is particularly difficult to treat owing to its resistance to radiation therapy. Here, we attempted to determine the key proteins responsible for melanoma radioresistance, with the aim of improving disease response to radiation therapy. Two melanoma cell lines, SK-Mel5 and SK-Mel28, with different radiosensitivities were analysed via RNA-Seq (Quant-Seq) and target proteins with higher abundance in the more radioresistant cell line, SK-Mel28, identified. Among these proteins, integrin αvβ3, a well-known molecule in cell adhesion, was selected for analysis. Treatment of SK-Mel28 cells with cilengitide, an integrin αvβ3 inhibitor, as well as γ-irradiation resulted in more significant cell death than γ-irradiation alone. In addition, Akt, a downstream signal transducer of integrin αvβ3, showed high basic activation in SK-Mel28 and was significantly decreased upon co-treatment with cilengitide and γ-irradiation. MK-2206, an Akt inhibitor, exerted similar effects on the SK-Mel28 cell line following γ-irradiation. Our results collectively demonstrate that the integrin αvβ3-Akt signalling pathway contributes to radioresistance in SK-Mel28 cells, which may be manipulated to improve therapeutic options for melanoma.

     


    Author information

    Im H1,2, Lee J1, Ryu KY2, Yi JY1.
    1
    Division of Radiation Biomedical Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
    2
    Department of Life Science, University of Seoul, Seoul, Korea.

  • 키워드
    MK-2206; SK-Mel28; SK-Mel5; cilengitide; γ-irradiation
  • 덧글달기
    덧글달기
       IP : 44.222.87.38

    등록