KIRAMS / 박인철*, 진현옥*
Abstract
BACKGROUND/AIM:
Phosphoserine aminotransferase 1 (PSAT1) is an enzyme implicated in serine biosynthesis, and its overexpression has been linked to cancer cell proliferation. Therefore, targeting PSAT1 is considered to be an anticancer strategy.
MATERIALS AND METHODS:
The viability of non-small cell lung cancer (NSCLC) cells was measured by MTT assay. Protein and mRNA expression were determined by western blot and reverse transcription polymerase chain reaction, respectively.
RESULTS:
Glutamine-limiting conditions were generated through glutamine deprivation or CB-839 treatment, which induced PSAT1 expression in NSCLC cells. PSAT1 expression induced by glutamine-limiting conditions was regulated by activating transcription factor 4. Knock-down of PSAT1 enhanced the sensitivity of NSCLC cells to glutamine-limiting conditions. Interestingly, ionizing radiation induced PSAT1 expression, and knocking down PSAT1 increased cell sensitivity to ionizing radiation.
CONCLUSION:
Inhibiting PSAT1 might aid in the treatment of lung cancer, and PSAT1 may be a therapeutic target for lung cancer.
Author information
Jin HO1, Hong SE2, Kim JY3, Jang SK2, Kim YS3, Sim JH3, Oh AC4, Kim H4, Hong YJ4, Lee JK3,4, Park IC5.
1
KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea hyeonok@kirams.re.kr parkic@kirams.re.kr.
2
Division of Fusion Radiology Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
3
KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
4
Department of Laboratory Medicine, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
5
Division of Fusion Radiology Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea hyeonok@kirams.re.kr parkic@kirams.re.kr.