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  • [Anticancer Res.] Knock-down of PSAT1 Enhances Sensitivity of NSCLC Cells to Glutamine-limiting Conditions.

    KIRAMS / 박인철*, 진현옥*

  • 출처
    Anticancer Res.
  • 등재일
    2019 Dec
  • 저널이슈번호
    39(12):6723-6730. doi: 10.21873/anticanres.13887.
  • 내용

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    Abstract
    BACKGROUND/AIM:
    Phosphoserine aminotransferase 1 (PSAT1) is an enzyme implicated in serine biosynthesis, and its overexpression has been linked to cancer cell proliferation. Therefore, targeting PSAT1 is considered to be an anticancer strategy.

    MATERIALS AND METHODS:
    The viability of non-small cell lung cancer (NSCLC) cells was measured by MTT assay. Protein and mRNA expression were determined by western blot and reverse transcription polymerase chain reaction, respectively.

    RESULTS:
    Glutamine-limiting conditions were generated through glutamine deprivation or CB-839 treatment, which induced PSAT1 expression in NSCLC cells. PSAT1 expression induced by glutamine-limiting conditions was regulated by activating transcription factor 4. Knock-down of PSAT1 enhanced the sensitivity of NSCLC cells to glutamine-limiting conditions. Interestingly, ionizing radiation induced PSAT1 expression, and knocking down PSAT1 increased cell sensitivity to ionizing radiation.

    CONCLUSION:
    Inhibiting PSAT1 might aid in the treatment of lung cancer, and PSAT1 may be a therapeutic target for lung cancer.

     


    Author information

    Jin HO1, Hong SE2, Kim JY3, Jang SK2, Kim YS3, Sim JH3, Oh AC4, Kim H4, Hong YJ4, Lee JK3,4, Park IC5.
    1
    KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea hyeonok@kirams.re.kr parkic@kirams.re.kr.
    2
    Division of Fusion Radiology Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
    3
    KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
    4
    Department of Laboratory Medicine, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
    5
    Division of Fusion Radiology Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea hyeonok@kirams.re.kr parkic@kirams.re.kr.

  • 키워드
    Activating transcription factor 4; glutamine; ionizing radiation; non-small cell lung cancer; phosphoserine aminotransferase 1
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