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Abstract
Cutaneous radiation injury (CRI) is a skin injury caused by high-dose exposure of ionizing radiation (IR). For proper treatment, early detection of CRI before clinical symptoms is important. Optical microscopic techniques such as reflectance confocal microscopy (RCM) and 2-photon microscopy (TPM) have been tested as the early diagnosis method by detecting cellular changes. In this study, RCM and TPM were compared in the detection of cellular changes caused by CRI in an in vivo mouse model. CRI was induced on the mouse hindlimb skin with various IR doses and the injured skin regions were imaged longitudinally by both modalities until the onset of clinical symptoms. Both RCM and TPM detected the changes of epidermal cells and sebaceous glands before clinical symptoms in different optical contrasts. RCM detected changes of cell morphology and scattering property based on light reflection. TPM detected detail changes of cellular structures based on autofluorescence of cells. Since both RCM and TPM were sensitive to the early stage CRI by using different contrasts, the optimal method for clinical CRI diagnosis could be either individual methods or their combination.

Author information

Jang WH1,2, Kwon S3, Shim S4, Jang WS5, Myung JK4,5,6, Yang S7,8, Park S4,5,6, Kim KH1,3.
1
Divison of Integrative Biosciences & Biotechnology, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea.
2
Biotech Center, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea.
3
Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea.
4
National Radiation Emergency Medical Centre, Korea Cancer Centre Hospital, Korea Institute of Radiological & Medical Sciences (KIRAMS), Seoul, South Korea.
5
Laboratory of Experimental Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological & Medical Sciences (KIRAMS), Seoul, South Korea.
6
Department of Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological & Medical Sciences (KIRAMS), Seoul, South Korea.
7
Medical Physics Division, Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, California.
8
Department of Electronics Engineering, Ewha Womans University, Seoul, South Korea.