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  • [Nutr Res Pract.] Radioprotective effects of delphinidin on normal human lung cells against proton beam exposure.

    건양대 / 김성진, 강보선*

  • 출처
    Nutr Res Pract.
  • 등재일
    2018 Feb
  • 저널이슈번호
    12(1):41-46. doi: 10.4162/nrp.2018.12.1.41. Epub 2018 Jan 18.
  • 내용

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    Abstract
    BACKGROUND/OBJECTIVES:
    Exposure of the normal lung tissue around the cancerous tumor during radiotherapy causes serious side effects such as pneumonitis and pulmonary fibrosis. Radioprotectors used during cancer radiotherapy could protect the patient from side effects induced by radiation injury of the normal tissue. Delphinidin has strong antioxidant properties, and it works as the driving force of a radioprotective effect by scavenging radiation-induced reactive oxygen species (ROS). However, no studies have been conducted on the radioprotective effect of delphinidin against high linear energy transfer radiation. Therefore, this study was undertaken to evaluate the radioprotective effects of delphinidin on human lung cells against a proton beam.

    MATERIALS/METHODS:
    Normal human lung cells (HEL 299 cells) were used for in vitro experiments. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay assessed the cytotoxicity of delphinidin and cell viability. The expression of radiation induced cellular ROS was measured by the 2'-7'-dicholordihydrofluorescein diacetate assay. Superoxide dismutase activity assay and catalase activity assay were used for evaluating the activity of corresponding enzymes. In addition, radioprotective effects on DNA damage-induced cellular apoptosis were evaluated by Western blot assay.

    RESULTS:
    Experimental analysis, including cell survival assay, MTT assay, and Western blot assay, revealed the radioprotective effects of delphinidin. These include restoring the activities of antioxidant enzymes of damaged cells, increase in the levels of pro-survival protein, and decrease of pro-apoptosis proteins. The results from different experiments were compatible with each to provide a substantial conclusion.

    CONCLUSION:
    Low concentration (2.5 µM/mL) of delphinidin administration prior to radiation exposure was radioprotective against a low dose of proton beam exposure. Hence, delphinidin is a promising shielding agent against radiation, protecting the normal tissues around a cancerous tumor, which are unintentionally exposed to low doses of radiation during proton therapy.

     


    Author information

    Kim HM1, Kim SH2, Kang BS1.
    1
    Department of Medical Science, Konyang University, 158 Gwanjeodong-ro, Seo-gu, Daejeon 35365, Korea.
    2
    Department of Food & Nutrition, Hyejeon College, Chungnam 32244, Korea.

  • 키워드
    Delphinidin; proton therapy; radiation protective agent; reactive oxygen species
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