방사선의학 웹진

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Abstract
Saengmaeksan (SMS), a representative oriental medicine that contains Panax ginseng Meyer, Liriope muscari, and Schisandra chinensis (1:2:1), is used to improve body vitality and enhance physical activity. However, there is limited scientific evidence to validate the benefits of SMS. Here, we investigated the in vitro and in vivo regulatory effects of SMS and its constituents on energy metabolism and the underlying molecular mechanisms. For this, quantitative real-time polymerase chain reaction, 3D holotomographic microscopy, western blotting, and glucose uptake experiments using 18F-fluoro-2-deoxy-D-glucose (18F-FDG) were performed using L6 cells to investigate in vitro energy metabolism changes. In addition, 18F-fluorocholine (18F-FCH) and 18F-FDG positron emission tomography/computed tomography (PET/CT) analyses, immunohistochemistry, and respiratory gas analysis were performed in mice post-endurance exercise on a treadmill. In the energy metabolism of L6 cells, a significant reversal in glucose uptake was observed in the SMS-treated group, as opposed to an increase in uptake over time compared to the untreated control group. Furthermore, P. ginseng alone and SMS significantly decreased the volume of lipid droplets. SMS also regulated the phosphorylation of extracellular signal-regulated kinase (ERK), phosphorylation of p38, mitochondrial morphology, and the expression of apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE/Ref-1) in H2O2-stimulated L6 cells. In addition, SMS treatment was found to regulate whole body and muscle energy metabolism in rats subjected to high-intensity exercise, as well as glucose and lipid metabolism in skeletal muscle. Therefore, SMS containing P. ginseng ameliorated imbalanced energy metabolism through oxidative stress-induced APE/Ref-1 expression. SMS may be a promising supplemental option for metabolic performance.

Affiliations

Suji Baek 1, Jisu Kim 2 3, Myung Hee Nam 4, Sun Mi Park 5, Tae Sup Lee 6, Seo Young Kang 5, Ji-Young Kim 5, Hai-Jeon Yoon 5, Seung Hae Kwon 4, Jonghoon Park 7, Sang Ju Lee 8, Seung Jun Oh 8, Kiwon Lim 2 3, Bom Sahn Kim 5, Kang Pa Lee 1, Byung Seok Moon 5
1Research & Development Center, UMUST R&D Corporation, Seoul, Korea.
2Physical Activity and Performance Institute (PAPI), Konkuk University, Seoul, Korea.
3Department of Sports Medicine and Science in Graduated School, Konkuk University, Seoul, Korea.
4Seoul Center, Korea Basic Science Institute, Seoul, Korea.
5Department of Nuclear Medicine, Ewha Womans University College of Medicine, Seoul, Korea.
6Division of RI Applications, Korea Institute Radiological and Medical Sciences, Seoul, Korea.
7Department of Physical Education, Korea University, Seoul, Korea.
8Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.