경북의대 / 손승현, Prakash Gangadaran,안병철*
Abstract
Background:
This study was designed to explore a novel approach for transferring NIS protein to cells using extracellular vesicle (EV) and enhancing iodine avidity in hepatocellular carcinoma (HCC) cells.
Methods:
We transfected the HCC cells (Huh7) with NIS gene, designated as Huh7/NIS, and isolated the EVs from them. Presence of NIS protein in EVs and EV-mediated transport of NIS protein to recipient Huh7 cells were tested using Western blotting. We also examined radioiodine uptake in Huh7 cells treated with EV-Huh7/NIS.
Results:
Successful transfer of NIS protein into Huh7 cells was confirmed by WB and microscopy. EVs showed high levels of NIS protein in them. Treatment of Huh7 cells with EV-Huh7/NIS increased the NIS protein level and enhanced 125I uptake in recipient Huh7 cells. In addition, EV-huh7/NIS pre-treatment enhanced the cytotoxicity of 131I therapy against Huh7 cells by inducing increased DNA damage/increased γH2A.X foci formation.
Conclusion:
This is the first-of-its-kind demonstration of successful transportation of the NIS protein to cells via EVs, which increased radioiodine uptake. This approach can revert radioiodine-resistant cancers into radioiodine-sensitive cancers.
Author information
Son SH1,2, Gangadaran P1,2, Ahn BC1,2.
1
Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea, abc2000@knu.ac.kr.
2
Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea, abc2000@knu.ac.kr.
편집위원
세포외 세포로 단백을 다른 세포론 전달되어 기능을 발휘할 수 있음을 보여준 연구임. 갑상선암에 NIS 단백이 방사성요오드 치료에 중요 요소인데, 세포외 세포로 NIS 단백을 간암에 전달하여 방사성요오드 축척능을 증가시킨 연구임. 분자영상 및 약물전달 연구에 관심이 있는 연구자에게 관심을 끌 수 있는 연구로 보임.
2019-04-17 15:59:36
편집위원2
세포외 소포(extracellular vesicles)를 이용하여 종양세포에 NIS (sodium iodide symporter) 단백을 전달하면 종양세포에 방사성요오드 축적을 증가시킬 수 있음을 보여준 연구임. 방사성요오드를 이용한 핵의학 치료를 연구하는 연구자 및 세포외 세포 관심 연구자에게 관심을 끌 수 있는 연구임.
2019-06-26 15:09:18