바로가기  >

Abstract

PURPOSE:
To investigate potential of chitosan hydrogel microparticles (CHI) for treatment of VX2carcinoma.

MATERIALS AND METHODS:
Two weeks after liver VX2 implantation, contrast-enhanced computerized tomographic scanning was conducted. Rabbits (n = 2) with successful tumor growth were treated with different sizes of 99mTc-labeled CHI (60-80 μm and 100-120 μm) via intra-arterial hepatic catheterization. Liver distribution of 99mTc-labeled CHI was determined by means of autoradiography, a radiation-based photographic technique. In the next part of this study, therapeutic effectiveness was examined with the use of CHI with the size range of 60-80 μm (n = 11). Tumor growth response and levels of blood liver enzymes were studied at baseline and 1 and 2 weeks after CHI treatment.

RESULTS:
Successful tumor growth was confirmed in all rabbits (24/24). Intrahepatic CHI with the size range of 60-80 μm resulted in liver localization in more close proximity to tumor nodule versus 100-120 μm. Baseline tumor volume was 1,909 ± 575 mm3 in animals receiving CHI versus 1,831 ± 249 mm3 in control animals (P = .342). In control animals, tumor volume markedly increased by 1,544 ± 512% at 2 weeks after sham operation versus baseline. In animals receiving CHI, tumorvolume remained relatively unchanged (54 ± 6% increase; P = .007 vs control). Levels of blood aspartate transaminase (AST) and alanine transaminase (ALT) in animals receiving CHI increased 1 week after treatment (P = .032 vs control for AST; P = .000 vs control for ALT), but returned to control levels at 2 weeks.

CONCLUSIONS:
CHI embolization suppressed tumor growth without appreciable damages in liverfunction.

Author information

Hwang H1, Kim HS1, Kwon J1, Oh PS1, Park HS2, Lim ST1, Sohn MH1, Jeong HJ3.

1 Department of Nuclear Medicine, Molecular Imaging and Therapeutic Medicine Research Center, Cyclotron Research Center, Research Institute of Clinical Medicine, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, 634-18 GeumAm-dong, Duckjin-gu, Jeonju-si, Jeollabuk-do 561-803, Republic of Korea.
2 Department of Pathology, Chonbuk National University Medical School and Hospital, Jeonju, Jeonbuk, Republic of Korea.
3 Department of Nuclear Medicine, Molecular Imaging and Therapeutic Medicine Research Center, Cyclotron Research Center, Research Institute of Clinical Medicine, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, 634-18 GeumAm-dong, Duckjin-gu, Jeonju-si, Jeollabuk-do 561-803, Republic of Korea. Electronic address: jayjeong@jbnu.ac.kr.