방사선의학 웹진

바로가기  >

그림1) Multimodal MR/Upconversion luminescent imaging of HCC tumor using transcatheter hepatic intra-arterial (IA) targeted anti-CD44-Nd-CSUCNPs. Multi-modal imaging reporters, Nd-CSUCNPs conjugated with an anti-CD44 monoclonal antibody, were delivered intra-arterially for the sensitive and rapid detection of HCC in orthotopic HCC rat models. IA delivery of multi-modality Nd-CSUCNPs conjugated with specific tumor targeting molecules facilitates rapid and selective targeting of Nd-CSUCNPs to HCC, providing strong image-contrast between the tumors and normal hepatic tissue. Next, the feasibility of multimodal diagnostic MR and intraoperative UCL image-guided surgery for HCC resection was demonstrated.

​그림2) An luminescent image of liver tumor in a HCC rat with 808 NIR laser and targeted anti-CD44 UCNPs.

Abstract

Multimodal-imaging probes offer a novel approach, which can provide detail diagnostic information for the planning of image-guided therapies in clinical practice. Here we report targeted multimodal Nd3+-doped upconversion nanoparticle (UCNP) imaging reporters, integrating both magnetic resonance imaging (MRI) and real-time upconversion luminescence imaging (UCL) capabilities within a single platform. Nd3+-doped UCNPs were synthesized as a core-shell structure showing a bright visible emission upon excitation at the near infrared (minimizing biological overheating and increasing tissue penetration depth) as well as providing strong MRI T2 contrast (high r2/r1 ratio). Transcatheter intra-arterial infusion of Nd3+-doped UCNPs conjugated with anti-CD44-monoclonal antibody allowed for high performance in vivo multimodal UCL and MR imaging of hepatocellular carcinoma (HCC) in an orthotopic rat model. The resulted in vivo multimodal imaging of Nd3+ doped core-shell UCNPs combined with transcatheter intra-arterial targeting approaches successfully discriminated liver tumors from normal hepatic tissues in rats for surgical resection applications. The demonstrated multimodal UCL and MRI imaging capabilities of our multimodal UCNPs reporters suggest strong potential for in vivo visualization of tumors and precise surgical guidance to fill the gap between pre-procedural imaging and intraoperative reality.

Author information

Lee J1, Gordon AC2, Kim H3, Park W4, Cho S4, Lee B5, Larson AC6, Rozhkova EA7, Kim DH8.​

1Center for Nanoscale Materials, Argonne National Laboratory, Argonne, IL 60439, USA.

2Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.

3Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.

4Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

5Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA.

6Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA; Robert H. Lurie Comprehensive Cancer Center, Chicago, IL 60611, USA; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA; Department of Electrical Engineering and Computer Science, Evanston, IL 60208, USA; International Institute of Nanotechnology (IIN), Northwestern University, Evanston, IL 60208, USA.

7Center for Nanoscale Materials, Argonne National Laboratory, Argonne, IL 60439, USA. Electronic address: rozhkova@anl.gov.

8Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA; Robert H. Lurie Comprehensive Cancer Center, Chicago, IL 60611, USA. Electronic address: dhkim@northwestern.edu.